Reliable and Remote Monitoring of Absolute Temperature During Liver Inflammation via Luminescence Lifetime-Based Nanothermometry

Temperature of tissues and organs is one of the first parameters affected by physiological and pathological processes, such as metabolic activity, acute trauma, or infection-induced inflammation. Therefore, the onset and development of these processes could be detected by monitoring deviations from basal temperature. To accomplish this, minimally invasive, reliable, and accurate measurement of the absolute temperature of internal organs is required. Luminescence nanothermometry is the ideal technology for meeting these requirements. Although this technique has lately undergone remarkable developments, its reliability is being questioned due to spectral distortions caused by biological tissues.
In this work, we demonstrate how the use of bright Ag2 S nanoparticles featuring temperature-dependent fluorescence lifetime enables reliable and accurate measurement of the absolute temperature of the liver in mice subjected to lipopolysaccharide induced inflammation. Beyond the remarkable thermal sensitivity (≈ 3% °C-1 around 37 °C) and thermal resolution obtained (smaller than 0.3°C), the results included in this work set a blueprint for the development of new diagnostic procedures based on the use of intra-corporeal temperature as a physiological indicator. This article is protected by copyright. All rights reserved.

Non-Interventional and High-Precision Temperature Measurement Biochips for Long-Term Monitoring the Temperature Fluctuations of Individual Cells

Monitoring the thermal responses of individual cells to external stimuli is essential for studies of cell metabolism, organelle function, and drug screening. Fluorescent temperature probes are usually employed to measure the temperatures of individual cells; however, they have some unavoidable problems, such as, poor stability caused by their sensitivity to the chemical composition of the solution and the limitation in their measurement time due to the short fluorescence lifetime. Here, we demonstrate a stable, non-interventional, and high-precision temperature-measurement chip that can monitor the temperature fluctuations of individual cells subject to external stimuli and over a normal cell life cycle as long as several days.
To improve the temperature resolution, we designed temperature sensors made of Pd-Cr thin-film thermocouples, a freestanding Si3N4 platform, and a dual-temperature control system. Our experimental results confirm the feasibility of using this cellular temperature-measurement chip to detect local temperature fluctuations of individual cells that are 0.3-1.5 K higher than the ambient temperature for HeLa cells in different proliferation cycles. In the future, we plan to integrate this chip with other single-cell technologies and apply it to research related to cellular heat-stress response.

Reliability of Low-Cost Thermometers for Monitoring Foot Temperature

The prevalence of diabetes in the United States and the world is high. Often the burden of this disease falls on those who are economically disadvantaged, contributing to disparities in access to care. Considering the risk for foot complications related to diabetes, foot temperature monitoring has been shown to be effective in mitigating risk for plantar ulceration.
Higher-cost thermometers have been assessed for reliability, while very low-cost thermometers have not. Validation of low-cost thermometers could improve access to this preventive measure. In this study, inexpensive commercially available infrared thermometers were shown to be a reliable and valid means to assess local skin temperature.

Modelling and Validation of a Guided Acoustic Wave Temperature Monitoring System

The computer modelling of condition monitoring sensors can aide in their development, improve their performance, and allow for the analysis of sensor impact on component operation. This article details the development of a COMSOL model for a guided wave-based temperature monitoring system, with a view to using the technology in the future for the temperature monitoring of nozzle guide vanes, found in the hot section of aeroengines. The model is based on an experimental test system that acts as a method of validation for the model.
Piezoelectric wedge transducers were used to excite the S0 Lamb wave mode in an aluminium plate, which was temperature controlled using a hot plate. Time of flight measurements were carried out in MATLAB and used to calculate group velocity. The results were compared to theoretical wave velocities extracted from dispersion curves. The assembly and validation of such a model can aide in the future development of guided wave based sensor systems, and the methods provided can act as a guide for building similar COMSOL models. The results show that the model is in good agreement with the experimental equivalent, which is also in line with theoretical predictions.

Ultra-high Sensitive Au-doped Silicon Nanomembrane Based Wearable Sensor Arrays for Continuous Skin Temperature Monitoring with High Precision

Monitoring the body temperature with high accuracy provides a fast, facile, but powerful route about the human body in a wide range of health information standards. Here, we introduce the first ever ultra-sensitive, and stretchable gold-doped silicon nanomembrane (Au-doped SiNM) epidermal temperature sensor array. The ultra-sensitivity is achieved by shifting freeze-out region to intrinsic region in carrier density and modulation of fermi energy level of p-type SiNM through the development of a novel gold-doping strategy.
The Au-doped SiNM is readily transferred onto an ultrathin polymer layer with a well-designed serpentine mesh structure, capable of being utilized as an epidermal temperature sensor array. Measurements in-vivo and in-vitro show temperature coefficient of resistance (TCR) as high as -37270.72ppm/°C, 22 times higher than existing metal-based temperature sensors with similar structures, and one of the highest thermal sensitivity among the inorganic material based temperature sensors. Applications in the continuous monitoring of body temperature and respiration rate during exercising (i.e., in a sweat environment) are demonstrated with a successful capture of information. This work lays a foundation for monitoring human body temperature, potentially useful for precision diagnosis (e.g., continuous monitoring body temperature in COVID-19 cases) and management of disease relevance to body and skin temperature in healthcare. This article is protected by copyright. All rights reserved.

Development of a time-temperature indicator based on Maillard reaction for visually monitoring the freshness of mackerel

Due to the highly cost-effective and maneuverable property, Maillard reaction-based time-temperature indicators (TTIs) are considered ideal devices for temperature track and quality indication. The objective of this study was to develop a cold-sensitive TTI based on the Maillard reaction reflecting the freshness of chilled seafood. Firstly, the color evolution trends of a series of Maillard reaction-based TTIs were investigated and the xylose-lysine group represented obvious color change.
Fourier transform infrared (FTIR) spectroscopy revealed the color change was associated with the formation of CN bond in melanoidin. Simultaneously, the relationships of the color change of TTI with time and temperature were established. The activation energy value (Ea) of TTI was close to that of mackerel. There existed a good relevance (R2 = 0.98) between the color change of TTI and the total volatile basic nitrogen content of mackerel, suggesting this novel TTI might have the potential to monitor the freshness of mackerel.

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High Temperature Requirement Factor A4 (HTRA4) Antibody

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